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KMID : 0375320000220020283
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Journal of Clinical Pathology and Quality Control
2000 Volume.22 No. 2 p.283 ~ p.286
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SDS-Agarose Gel Electrophoresis Patterns of Urine Protein and Serum Protein Electrophoresis Patterns in the Diabetic Nephropathies
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Kwon Hi-Jeong
Min Ki-Ouk
Seo Eun-Joo
Kim Byung-Kee
Ahn Seog-Joo
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Abstract
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Background : We evaluated serum protein electrophoresis and urine SDS-agarose gel electrophoresis(SDS-AGE) for protein in diabetic nephoropathy patients to assess their diagnostic and prognostic value.
Methods : Fifty four cases of diabetic nephropathy (mean age, 60.9 (31-75) years; male, n=28; female, n=26) and control group(n=1 0) were studied. Random urine was analyzed by SDS-AGE. These were determined from the presence of low molecular weight and/or high molecular weight proteins by SDS-AGE. Serum protein electrophoresis, total protein, albumin, BUN, creatinine were measured.
Results : The patterns of proteinuria were glomerular(n=11), tubular(n=2), mixed(n=16), mixedmostly glomerular(n=10), mixed-mostly tubular(n=2), albumin only(n=13). Nine cases showed nephrotic syndrome pattern by serum protein electrophoresis. Mean serum total protein was 6.93 ±0.84 g/dL; albumin, 3.91 ±0.56 g/dL; BUN, 26.8 ±17.74 mg/dL; creatinine, 1.52±1.02 mg/dL. Compared to the control group, the albumin level of diabetic nephropathies was low, but the alpha 1 and alpha 2 globulin levels were high. A/G ratio was low and beta and gamma fractions showed no differences.
Conclusions : Using SDS-AGE of proteinuria, various kidney damage patterns were observed. There were no specific findings in diabetic nephropathies by serum protein electrophoresis.
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KEYWORD
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Diabetic nephropathy, SDS-AGE, Electrophoresis
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